What is analysed?

Anti-double stranded DNA (anti-dsDNA) are antibodies that target double-stranded DNA molecules. The presence of anti-dsDNA serves as a marker for systemic lupus erythematosus (SLE) and autoimmune hepatitis, two chronic autoimmune disorders.

What does the result mean?

The result indicates the presence or absence of anti-dsDNA in the blood.

Why conduct the analysis?

The anti-dsDNA test is conducted to help diagnose SLE and autoimmune hepatitis.

When to conduct the analysis?

The anti-dsDNA test may be conducted when symptoms such as joint pain, skin rashes, or fatigue are observed. In addition, the analysis may also be conducted during treatment for SLE or autoimmune hepatitis to evaluate the effectiveness of treatment or manage flare-ups.

What sample is required?

A blood sample is used for the analysis of anti-dsDNA. The blood is drawn from a vein, usually in the arm.

Is any prior preparation necessary?

Patients should fast for 8-12 hours before the blood sample is taken. In addition, patients should also inform a healthcare professional about any medications being taken, as they could affect results.

How is it performed?

The collected sample is sent to a laboratory, where it is analysed using enzyme-linked immunosorbent assay (ELISA) and Crithidia luciliae immunofluorescence for the presence of anti-dsDNA.

What are the normal values?

Between 10-15 IU/mL is seen as a normal or negative anti-dsDNA test result. 

More than 15 IU/mL is seen as a positive anti-dsDNA test result.

What does having altered values mean?

• Positive result: A positive test result indicates the presence of anti-dsDNA antibodies, suggesting SLE. However, a positive identification alone isn't a sure sign of SLE. Further tests and a clinical evaluation are required.