What is being analysed?

The Direct Antiglobulin Test, or DAT, is a laboratory test that evaluates whether red blood cells (RBCs) have antibodies or complement proteins attached to their surface.

What does the result mean?

A positive result indicates the presence of antibodies or complement proteins on the surface of red blood cells. This may suggest immune-mediated haemolytic anaemia or other immune-related disorders.

Why perform the analysis?

The DAT is crucial in diagnosing conditions where the immune system attacks and destroys red blood cells, helping healthcare professionals determine the underlying cause of haemolysis.

When to conduct the analysis?

The test is typically ordered when a patient exhibits symptoms of haemolytic anaemia, such as jaundice, fatigue, or dark urine. It is also performed in cases of blood transfusion reactions and certain autoimmune disorders.

What sample is required?

A small sample of blood is collected from the patient through a standard blood draw. This blood sample is then sent to the laboratory for analysis.

Is any prior preparation necessary?

No special preparation is usually required for the Direct Antiglobulin Test. However, it is essential to inform your healthcare provider about any medications or medical conditions you may have.

How is it performed?

In the laboratory, the collected blood sample is mixed with specific reagents that can detect the presence of antibodies or complement proteins on the surface of red blood cells. The reaction is then observed under a microscope or analysed through other techniques.

What are the normal values?

What does it mean to have altered values?

Altered values, indicating a positive result, suggest an immune response against red blood cells. This could be due to autoimmune disorders, drug-induced reactions, or transfusion-related complications. Further diagnostic tests and evaluation by a healthcare professional are essential for accurate diagnosis and appropriate treatment.